nm 002168 Search Results


human idh2 cdna  (OriGene)
90
OriGene human idh2 cdna
Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or <t>IDH2(R172K)</t> from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) <t>complementary</t> <t>DNA.</t> f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.
Human Idh2 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nm+002168/pm25043045-287-8-11?v=OriGene
Average 90 stars, based on 1 article reviews
human idh2 cdna - by Bioz Stars, 2026-08
90/100 stars
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pcmv6 idh2 wild type plasmid  (OriGene)
90
OriGene pcmv6 idh2 wild type plasmid
Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or <t>IDH2(R172K)</t> from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) <t>complementary</t> <t>DNA.</t> f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.
Pcmv6 Idh2 Wild Type Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nm+002168/pmc08488436-44-1-5?v=OriGene
Average 90 stars, based on 1 article reviews
pcmv6 idh2 wild type plasmid - by Bioz Stars, 2026-08
90/100 stars
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idh2 gfp construct  (OriGene)
93
OriGene idh2 gfp construct
Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or <t>IDH2(R172K)</t> from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) <t>complementary</t> <t>DNA.</t> f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.
Idh2 Gfp Construct, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nm+002168/pmc04821487__ja5b07627_si_001-39-24-26?v=OriGene
Average 93 stars, based on 1 article reviews
idh2 gfp construct - by Bioz Stars, 2026-08
93/100 stars
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hidh2wild type wt myc flag  (OriGene)
90
OriGene hidh2wild type wt myc flag
Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or <t>IDH2(R172K)</t> from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) <t>complementary</t> <t>DNA.</t> f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.
Hidh2wild Type Wt Myc Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nm+002168/10__1096_slash_fj__201800788r-61-43-46?v=OriGene
Average 90 stars, based on 1 article reviews
hidh2wild type wt myc flag - by Bioz Stars, 2026-08
90/100 stars
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idh2 r172k orf in pcmv6  (OriGene)
90
OriGene idh2 r172k orf in pcmv6

Idh2 R172k Orf In Pcmv6, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nm+002168/pmc04786416-4-0-8?v=OriGene
Average 90 stars, based on 1 article reviews
idh2 r172k orf in pcmv6 - by Bioz Stars, 2026-08
90/100 stars
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idh2 nm 002168 human tagged orf clone  (OriGene)
N/A
Lenti ORF clone of Human isocitrate dehydrogenase 2 NADP mitochondrial IDH2 nuclear gene encoding mitochondrial protein Myc DDK tagged
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idh2 nm 002168 human recombinant protein  (OriGene)
N/A
Purified recombinant protein of mutant R172M of Homo sapiens isocitrate dehydrogenase 2 NADP mitochondrial IDH2 with C terminal DDK tag expressed in sf9 cells 20 µg
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Image Search Results


Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or IDH2(R172K) from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) complementary DNA. f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.

Journal: Nature

Article Title: Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer.

doi: 10.1038/nature13441

Figure Lengend Snippet: Figure 2 | Mutant IDH blocks hepatocyte differentiation by silencing HNF- 4a. a, Heat map of GSEA showing top-ranked gene sets distinguishing IDH1(R132C) or IDH2(R172K) from wild-type (WT) or empty vector (EV) control hepatoblasts (pairwise analysis; replicates for each condition; see Methods). NES, normalized enrichment score; NS, not significant. b, c, Hepatoblasts analysed by immunoblot (b) and qRT–PCR (c). d, e, Analysis of wild-type hepatoblasts expressing the indicated short hairpin (sh)RNAs (uncoated plates). CTL, control. d, Hepatocyte sphere formation. e, Proliferation of shRNA-expressing hepatoblast cells co-expressing EV or shRNA-resistant Hnf4a(1) complementary DNA. f–h, Control and R132C- expressing hepatoblasts co-expressing vector control (EV2) or HNF-4a, grown on uncoated plates. f, Hepatocyte sphere formation. g, Hepatocyte gene expression. h, Proliferation. Scale bars, 100mm (d), 250mm (f). *P , 0.05.

Article Snippet: The R140Q and R172K mutations were introduced into human IDH2 cDNA (Origene, MD # SC319226, NCBI RefSeq accession number NM_002168.3) using the Quickchange kit (Agilent Lexington, MA #200521) as per the manufacturer’s instructions.

Techniques: Mutagenesis, Plasmid Preparation, Control, Western Blot, Quantitative RT-PCR, Expressing, shRNA, Gene Expression

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Modification, Plasmid Preparation, Protein Extraction, Protease Inhibitor, Spectrophotometry, DC Protein Assay, Staining

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Modification, Plasmid Preparation, Generated

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Plasmid Preparation

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Mutagenesis

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

Figure Lengend Snippet:

Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Mutagenesis